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Order before 2pm ET — ships from Toronto
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Cold-pack shipped
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Purity guarantee
Below spec? Replaced or refunded
NAD+
The Cellular Energy Coenzyme at the Centre of Longevity Research
NAD+ (nicotinamide adenine dinucleotide) is a coenzyme present in every living cell and is essential to mitochondrial energy production, DNA repair via PARP enzymes, and sirtuin-mediated longevity signalling. Tissue NAD+ concentrations decline substantially with age — human skin and plasma studies report reductions of up to 50% between early adulthood and middle age — which has made NAD+ restoration one of the most actively investigated targets in modern longevity science. Trinity Labs supplies lyophilized NAD+ disodium salt at ≥99% HPLC purity for in-vitro cellular metabolism, mitochondrial function, and senescence research.
- Central coenzyme for mitochondrial ATP production, PARP DNA repair and sirtuin signalling
- Tissue NAD+ declines up to ~50% between early adulthood and midlife — the core premise of restoration research
- ≥99% HPLC purity disodium salt, batch-matched COA with every 500mg vial
500mg per vial · Lyophilized Powder · Ships same business day from Toronto
Questions about this batch? Text an agent at (905) 993-4764
99.3%
HPLC purity
Batch TR-NAD-2026-001, ISO 17025 verified
~50%
Age-related decline
Reported reduction in tissue NAD+ from young adulthood to midlife
7
Sirtuins NAD+-dependent
SIRT1–SIRT7 all require NAD+ as an obligate substrate
Research benefits
Mitochondrial Energy
Essential redox cofactor for the electron transport chain and ATP production
DNA Repair & Sirtuins
Required substrate for PARP1 DNA repair enzymes and SIRT1–SIRT7 longevity signalling
Neuro & Cognitive Models
Studied for neuronal resilience, axonal protection and cognitive markers in aging models
Study findings
Summaries of published research on this compound. Findings are reported for scientific context only and do not constitute claims about this product.
NAD+ repletion improves mitochondrial and stem cell function
Restoring NAD+ with nicotinamide riboside rejuvenated muscle stem cells, improved mitochondrial function and extended lifespan in aged mice.
Science · 2016
Declining NAD+ induces a pseudohypoxic state
Age-related NAD+ decline disrupted nuclear–mitochondrial communication via SIRT1, and raising NAD+ reversed the mitochondrial deficit within one week in mice.
Cell · 2013
NAD+ metabolism in aging and disease
Comprehensive review establishing NAD+ as a shared node across metabolic, neurodegenerative and cardiovascular aging phenotypes.
Cell Metabolism · 2018
Skin NAD+ measurements across the lifespan
Human skin biopsy analysis showed a progressive, significant decline in NAD+ concentration with chronological age.
Nature Communications · 2019
PARP1 activity consumes NAD+ during DNA repair
PARP1 activation in response to DNA damage is a principal cellular NAD+ sink, linking genomic stress directly to NAD+ availability.
Molecular Cell · 2017
What researchers report
Aggregated, anonymized observations from laboratories ordering this compound. Anecdotal and non-clinical.
Most frequently ordered for mitochondrial respiration and senescence assays rather than single-endpoint models.
Researchers commonly report the disodium salt dissolves fully and clear within seconds of gentle swirling.
Often paired with MOTS-c in energy-metabolism study arms because the two act on complementary pathways.
Light sensitivity is the most-cited handling note; amber storage is regularly used for reconstituted aliquots.
Technical specifications
| Form | Lyophilized Powder |
| Sequence | Nicotinamide adenine dinucleotide (disodium salt) |
| Molecular Weight | 663.43 g/mol |
| Purity | ≥99% |
| Amount | 500mg per vial |
| Storage | Store at -20°C, protect from light |
| Shelf Life | 24 months (stored at -20°C) |
| COA | COA Available → View Lab Results |
Storage & reconstitution
Store lyophilized vial at -20°C, protected from light — NAD+ is notably photosensitive.
Reconstitute with bacteriostatic water; swirl gently and avoid vigorous shaking.
Refrigerate reconstituted solution at 2–8°C, keep in amber or foil-wrapped vials, and use within 14–30 days.
Avoid repeated freeze-thaw cycles; aliquot before freezing if long-term storage is required.
Research background
NAD+ was first identified in 1906 as a fermentation cofactor and is now recognised as a central hub of cellular metabolism and repair.
Human tissue NAD+ levels decline markedly with age; studies in skin, liver and brain tissue report reductions approaching 50% by midlife.
Sirtuin and PARP enzyme activity is NAD+-dependent, linking NAD+ availability directly to DNA repair capacity and metabolic regulation.
Frequently stacked with
MOTS-c
Mitochondrial-derived peptide for AMPK, metabolism and healthspan research.
COA Available
GHK-Cu
Copper tripeptide complex for skin remodeling & longevity research.
COA AvailableThird-party verified
ISO 17025 accredited lab — Batch TR-NAD-2026-001
HPLC Purity
99.3%
Molecular Weight
✓ Verified
Endotoxin (LAL)
<1.0 EU/mg
Heavy Metals (ICP-MS)
✓ Pass
NAD+ — frequently asked
Is this NAD+ or a precursor like NMN/NR?
This is NAD+ itself, supplied as the disodium salt — not a precursor that must be converted intracellularly.
Why 500mg per vial?
NAD+ research protocols use substantially larger quantities than peptide models, so a 500mg vial matches typical multi-arm study designs.
Why does the solution turn slightly yellow?
NAD+ oxidises on exposure to light and air. Protect from light and refrigerate; discoloured solution should be discarded.
What purity do you guarantee?
≥99% by HPLC, with a batch-matched third-party COA including heavy metals (ICP-MS) and endotoxin results.
How fast does it ship?
Orders placed before 2pm ET ship the same business day from Toronto with tracked Canada-wide delivery, typically arriving in 1–3 business days. Cold-pack packaging is used on every shipment.
What if the purity doesn't match the COA?
Every batch is HPLC and mass-spec verified by an ISO 17025 accredited third-party lab, and the batch-matched COA ships with your order. If an independent test comes back below the stated purity, we replace the order or refund it in full.
Is this legal to purchase in Canada?
Yes — these compounds are sold strictly as in-vitro research chemicals for laboratory use. They are not for human or veterinary use, and it is the purchaser's responsibility to comply with applicable federal and provincial regulations.
Ready to add NAD+ to your research?
≥99% HPLC-verified, endotoxin and heavy metals tested, batch-matched COA included. Ships same business day from Toronto.
Research Use Only Disclaimer
This product is sold exclusively for in-vitro research and laboratory use only. It is NOT intended for human or veterinary use, consumption, injection, or any clinical application. This product has not been evaluated by Health Canada, the FDA, or any other regulatory authority for safety or efficacy in humans or animals. Trinity makes no medical claims regarding this compound. Researchers must comply with all applicable federal, provincial/state, and local laws regarding the purchase, storage, and use of research compounds. By purchasing this product, you confirm you are a qualified researcher or licensed professional acting within the bounds of applicable law.